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BMRB: 50356

7BBB

1H, 13C, 15N backbone and ILMVAT methyl group assignment of the C-terminal RecA and RBD domains of the DEAD-box RNA helicase DbpA from E.coli

Authors

Wurm, J.

Assembly

C-terminal RecA domain + RBD of DbpA

Entity

1. C-terminal RecA domain + RBD of DbpA (polymer, Thiol state: all free), 251 monomers, 26976.70 Da Detail

GGSTDALPPI EQQFYETSSK GKIPLLQRLL SLHQPSSCVV FCNTKKDCQA VCDALNEVGQ SALSLHGDLE QRDRDQTLVR FANGSARVLV ATDVAARGLD IKSLELVVNF ELAWDPEVHV HRIGRTARAG NSGLAISFCA PEEAQRANII SDMLQIKLNW QTPPANSSIA TLEAEMATLC IDGGKKAKMR PGDVLGALTG DIGLDGADIG KIAVHPAHVY VAVRQAVAHK AWKQLQGGKI KGKTCRVRLL K

Formula weight

26976.7 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 54.5 %, Completeness (bb): 78.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.5 % (1543 of 2833)	32.9 % (480 of 1460)	74.1 % (810 of 1093)	90.4 % (253 of 280)
Backbone	78.2 % (1162 of 1486)	48.0 % (246 of 513)	93.4 % (684 of 732)	96.3 % (232 of 241)
Sidechain	38.0 % (599 of 1577)	24.7 % (234 of 947)	58.2 % (344 of 591)	53.8 % (21 of 39)
Aromatic	16.9 % (22 of 130)	24.6 % (16 of 65)	4.8 % (3 of 62)	100.0 % (3 of 3)
Methyl	90.0 % (288 of 320)	90.0 % (144 of 160)	90.0 % (144 of 160)

1. entity 1

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	sodium chloride	natural abundance	250 mM
2	HEPES	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	C-terminal RecA domain + RNA binding domain	[U-13C; U-15N; U-2H; 95% 1HD-Ile,Leu; 95% 1HG-Val,95% 1H,13CE-Met]	750 uM
5	Arginine	natural abundance	25 mM
6	Glutamate	natural abundance	25 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Concentration
7	sodium chloride	natural abundance	250 mM
8	HEPES	natural abundance	25 mM
9	DTT	natural abundance	1 mM
10	C-terminal RecA domain + RNA binding domain	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met, 1H13CG-Thr,U-1H-Tyr]	800 uM
11	Arginine	natural abundance	25 mM
12	Glutamate	natural abundance	25 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Concentration
13	sodium chloride	natural abundance	250 mM
14	HEPES	natural abundance	25 mM
15	DTT	natural abundance	1 mM
16	C-terminal RecA domain + RNA binding domain	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met]	800 uM
17	Arginine	natural abundance	25 mM
18	Glutamate	natural abundance	25 mM

Release date

2020-06-24

Citation

Assignment of the Ile, Leu, Val, Met and Ala methyl group resonances of the DEAD-box RNA helicase DbpA from E. coli
Wurm, J.
Biomol. NMR Assign. (2021), 15, 121-128, PubMed 33277687 , DOI 10.1007/s12104-020-09994-z , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 50355 released on 2020-06-24
    Title 1H, 13C, 15N backbone and ILMVA methyl group assignment of the N-terminal RecA domain of the DEAD-box RNA helicase DbpA from E.coli
  BMRB: 50357 released on 2020-06-24
    Title 1H, 15N backbone and ILMVA methyl group assignment of the DEAD-box RNA helicase DbpA from E.coli

Related entities 1. C-terminal RecA domain + RBD of DbpA, : 1 : 5 : 5 entities Detail

Interaction partners 1. C-terminal RecA domain + RBD of DbpA, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC