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BMRB: 5105


5105
1RYK
An NMR Approach to Structural Proteomics
Authors
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Assembly
EC298
Entity
1. EC298 (polymer, Thiol state: not present), 69 monomers, 8325.146 Da Detail

MNKDEAGGNW KQFKGKVKEQ WGKLTDDDMT IIEGKRDQLV GKIQERYGYQ KDQAEKEVVD WETRNEYRW


Formula weight
8325.146 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.6 %, Completeness: 77.6 %, Completeness (bb): 82.9 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All77.6 % (667 of 860)83.9 % (385 of 459)67.7 % (216 of 319)80.5 % (66 of 82)
Backbone82.9 % (343 of 414)97.2 % (141 of 145)68.0 % (136 of 200)95.7 % (66 of 69)
Sidechain75.8 % (385 of 508)77.7 % (244 of 314)77.9 % (141 of 181) 0.0 % (0 of 13)
Aromatic 0.0 % (0 of 82) 0.0 % (0 of 41) 0.0 % (0 of 37) 0.0 % (0 of 4)
Methyl97.8 % (45 of 46)100.0 % (23 of 23)95.7 % (22 of 23)

1. EC298

MNKDEAGGNW KQFKGKVKEQ WGKLTDDDMT IIEGKRDQLV GKIQERYGYQ KDQAEKEVVD WETRNEYRW

Show sample condition
Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
1EC298[U-13C; U-15N]1.0 mM

Release date
2002-04-07
Citation
An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 ,
Entries sharing articles BMRB: 7, Swiss-Prot: 4 entries Detail
  BMRB: 5051 released on 2002-08-21
    Title Backbone 1H, 13C, and 15N and Side-Chain 1H Chemical Shift Assignments for MTH1692
  BMRB: 5059 released on 2002-08-21
    Title Chemical shift assignments for EC005 from E. coli
  BMRB: 5077 released on 2002-05-27
    Title 1H, 15N, 13C NMR Assignments of M156R Protein from myxoma virus, NESG target OP2
  BMRB: 5165 released on 2002-05-05
    Title Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598
  BMRB: 5104 released on 2002-04-07
    Title An NMR Approach to Structural Proteomics
  BMRB: 5106 released on 2002-04-07
    Title An NMR Approach to Structural Proteomics
  BMRB: 5166 released on 2001-10-17
    Title Solution structure of hemolysin expression modulating protein Hha
  Swiss-Prot: P0ACE3 released on 2005-11-22
    Title HHA_ECOLI Entity Hemolysin expression-modulating protein Hha
  Swiss-Prot: P0AA31 released on 2005-09-13
    Title YEDF_ECOLI Entity UPF0033 protein YedF
  Swiss-Prot: O27635 released on 2002-09-19
    Title ARCH_METTH Entity Protein archease
  Swiss-Prot: Q9X078 released on 2001-02-21
    Title Y983_THEMA Entity UPF0033 protein TM_0983
Related entities 1. EC298, : 1 : 2 : 27 entities Detail
More details for 30 related entities
Interaction partners 1. EC298, : 8 interactors Detail
More details for 8 interactors identified by 4 citations
Chemical shift validation 3 contents Detail