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An NMR Approach to Structural Proteomics
Authors
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Assembly
MTH1743
Entity
1. MTH1743 (polymer, Thiol state: not present), 70 monomers, 7755.993 Da Detail

MVIGMKFTVI TDDGKKILES GAPRRIKDVL GELEIPIETV VVKKNGQIVI DEEEIFDGDI IEVIRVIYGG


Formula weight
7755.993 Da
Source organism
Methanothermobacter thermautotrophicus
Exptl. method
NMR
Refine. method
torsion angle dyanmics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 94.4 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All94.4 % (777 of 823)95.8 % (409 of 427)92.3 % (301 of 326)95.7 % (67 of 70)
Backbone97.4 % (405 of 416)97.9 % (143 of 146)97.0 % (196 of 202)97.1 % (66 of 68)
Sidechain92.3 % (433 of 469)94.7 % (266 of 281)89.2 % (166 of 186)50.0 % (1 of 2)
Aromatic35.7 % (10 of 28)71.4 % (10 of 14) 0.0 % (0 of 14)
Methyl98.1 % (106 of 108)100.0 % (54 of 54)96.3 % (52 of 54)

1. MTH1743

MVIGMKFTVI TDDGKKILES GAPRRIKDVL GELEIPIETV VVKKNGQIVI DEEEIFDGDI IEVIRVIYGG

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
1MTH1743[U-13C; U-15N]0.0 ~ 0.0 mM

LACS Plot; CA
Referencing offset: -0.19 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.19 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.08 ppm, Outliers: 5 Detail
LACS Plot; CO
Referencing offset: 0.43 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1RYJ, Strand ID: A Detail


Release date
2002-04-07
Citation
An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 ,
Entries sharing articles BMRB: 7, Swiss-Prot: 4 entries Detail
  BMRB: 5051 released on 2002-08-21
    Title Backbone 1H, 13C, and 15N and Side-Chain 1H Chemical Shift Assignments for MTH1692
  BMRB: 5059 released on 2002-08-21
    Title Chemical shift assignments for EC005 from E. coli
  BMRB: 5077 released on 2002-05-27
    Title 1H, 15N, 13C NMR Assignments of M156R Protein from myxoma virus, NESG target OP2
  BMRB: 5165 released on 2002-05-05
    Title Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598
  BMRB: 5104 released on 2002-04-07
    Title An NMR Approach to Structural Proteomics
  BMRB: 5105 released on 2002-04-07
    Title An NMR Approach to Structural Proteomics
  BMRB: 5166 released on 2001-10-17
    Title Solution structure of hemolysin expression modulating protein Hha
  Swiss-Prot: P0ACE3 released on 2005-11-22
    Title HHA_ECOLI Entity Hemolysin expression-modulating protein Hha
  Swiss-Prot: P0AA31 released on 2005-09-13
    Title YEDF_ECOLI Entity UPF0033 protein YedF
  Swiss-Prot: O27635 released on 2002-09-19
    Title ARCH_METTH Entity Protein archease
  Swiss-Prot: Q9X078 released on 2001-02-21
    Title Y983_THEMA Entity UPF0033 protein TM_0983
Related entities 1. MTH1743, : 1 : 26 entities Detail
Experiments performed 9 experiments Detail
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