BMRB: 5166

Solution structure of hemolysin expression modulating protein Hha

Authors

Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.

Assembly

hemolysin expression modulating protein Hha

Entity

1. hemolysin expression modulating protein Hha (polymer, Thiol state: all free), 72 monomers, 8627.901 Da Detail

MSEKPLTKTD YLMRLRRCQT IDTLERVIEK NKYELSDNEL AVFYSAADHR LAELTMNKLY DKIPSSVWKF IR

Formula weight

8627.901 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 76.0 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	76.0 % (679 of 893)	78.7 % (369 of 469)	69.6 % (243 of 349)	89.3 % (67 of 75)
Backbone	81.3 % (348 of 428)	95.1 % (135 of 142)	69.0 % (149 of 216)	91.4 % (64 of 70)
Sidechain	74.9 % (402 of 537)	71.6 % (234 of 327)	80.5 % (165 of 205)	60.0 % (3 of 5)
Aromatic	25.0 % (17 of 68)	41.2 % (14 of 34)	6.1 % (2 of 33)	100.0 % (1 of 1)
Methyl	98.8 % (81 of 82)	100.0 % (41 of 41)	97.6 % (40 of 41)

1. hemolysin expression modulating protein

MSEKPLTKTD YLMRLRRCQT IDTLERVIEK NKYELSDNEL AVFYSAADHR LAELTMNKLY DKIPSSVWKF IR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hemolysin expression modulating protein	[U-15N; U-13C]		2 mM
2	NaCl			150 mM

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LACS Plot; CA

Referencing offset: -0.59 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.59 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 10 models in PDB: 1JW2, Strand ID: A Detail

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Release date

2001-10-17

Citation

An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 , Abstract

Entries sharing articles BMRB: 7, Swiss-Prot: 4 entries Detail

Related entities 1. hemolysin expression modulating protein Hha, : 1 : 3 : 7 entities Detail

Experiments performed 8 experiments Detail