Native State Hydrogen Exchange Study of Suppressor and Pathogenic Variants of Transthyretin
MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGELHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTM AVVTNPKE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 27.6 % (396 of 1433) | 18.8 % (138 of 734) | 24.2 % (139 of 574) | 95.2 % (119 of 125) |
Backbone | 50.1 % (377 of 752) | 47.7 % (123 of 258) | 36.6 % (137 of 374) | 97.5 % (117 of 120) |
Sidechain | 3.1 % (25 of 799) | 3.2 % (15 of 476) | 2.5 % (8 of 318) | 40.0 % (2 of 5) |
Aromatic | 3.1 % (4 of 130) | 3.1 % (2 of 65) | 0.0 % (0 of 63) | 100.0 % (2 of 2) |
Methyl | 0.0 % (0 of 142) | 0.0 % (0 of 71) | 0.0 % (0 of 71) |
1. Transthyretin
MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGELHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTM AVVTNPKETemperature 298 (±2) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Transthyretin | [U-C13; U-N15] | 0.3 ~ 1.5 mM | |
2 | D2O | 100 % |