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BMRB: 17897


17897
2LYR
NOE-based 3D structure of the monomeric partially-folded intermediate of CylR2 at 259K (-14 Celsius degrees)
Authors
Jaremko, M., Jaremko, L., Kim, H., Cho, M., Schwieters, C.D., Giller, K., Becker, S., Zweckstetter, M.
Assembly
CylR2
Entity
1. CylR2 (polymer, Thiol state: not present), 66 monomers, 7715.914 Da Detail

MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPE


Formula weight
7715.914 Da
Exptl. method
solution NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 65.8 %, Completeness (bb): 66.2 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All65.8 % (553 of 841)67.9 % (302 of 445)56.7 % (182 of 321)92.0 % (69 of 75)
Backbone66.2 % (258 of 390)86.2 % (112 of 130)44.7 % (88 of 197)92.1 % (58 of 63)
Sidechain61.4 % (317 of 516)59.7 % (188 of 315)63.0 % (119 of 189)83.3 % (10 of 12)
Aromatic43.5 % (20 of 46)43.5 % (10 of 23)45.5 % (10 of 22) 0.0 % (0 of 1)
Methyl97.7 % (86 of 88)97.7 % (43 of 44)97.7 % (43 of 44)

1. CylR2

MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPE

Show sample condition
Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 259 K, pH 7.0, Details sample was in 10 capillaries ca. 25-30 uL, each inserted into a 5mm NMR tube


#NameIsotope labelingTypeConcentration
1entity[U-100% 13C; U-100% 15N]0.52 (±0.05) mM
2sodium chloridenatural abundance600 mM
3HEPESnatural abundance50 mM
4H2Onatural abundance93 %
5D2Onatural abundance7 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2LYR, Strand ID: A Detail

Release date
2013-02-14
Citation
Cold denaturation of a protein dimer monitored at atomic resolution
Jaremko, M., Jaremko, L., Kim, H., Cho, M., Schwieters, C.D., Giller, K., Becker, S., Zweckstetter, M.
Nat. Chem. Biol. (2013), 9, 264-270, PubMed 23396077 , DOI 10.1038/nchembio.1181 ,
Entries sharing articles BMRB: 6 entries Detail
  BMRB: 17892 released on 2013-02-14
    Title NOE-based 3D structure of the CylR2 homodimer at 298K
  BMRB: 17893 released on 2013-02-14
    Title NOE-based 3D structure of the CylR2 homodimer at 270K (-3 Celsius degrees)
  BMRB: 17894 released on 2013-02-14
    Title NOE-based 3D structure of the predissociated homodimer of CylR2 in equilibrium with monomer at 266K (-7 Celsius degrees)
  BMRB: 17895 released on 2013-02-14
    Title NOE-based 3D structure of the monomer of CylR2 in equilibrium with predissociated homodimer at 266K (-7 Celsius degrees)
  BMRB: 17896 released on 2013-02-14
    Title NOE-based 3D structure of the monomeric intermediate of CylR2 at 262K (-11 Celsius degrees)
  BMRB: 17898 released on 2013-02-14
    Title NOE-based 3D structure of the monomeric partially-folded intermediate of CylR2 at 257K (-16 Celsius degrees)
Related entities 1. CylR2, : 1 : 9 : 2 : 58 entities Detail
More details for 70 related entities
Experiments performed 7 experiments Detail
nullKeywords cold denaturation, CylR2, cytolysin repressor 2, DNA-binding protein, helix-turn-helix, NOE-based structure, partially folded, protein folding