NMR Assignment and structure of CssA5 (middle region) of CssA thermometer from Neisseria meningitidis
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 36.3 % (250 of 689) | 53.6 % (200 of 373) | 11.0 % (32 of 292) | 75.0 % (18 of 24) |
Suger, PO4 | 19.7 % (93 of 473) | 36.0 % (93 of 258) | 0.0 % (0 of 215) | |
Nucleobase | 72.7 % (157 of 216) | 93.0 % (107 of 115) | 41.6 % (32 of 77) | 75.0 % (18 of 24) |
Aromatic | 71.8 % (145 of 202) | 94.1 % (95 of 101) | 41.6 % (32 of 77) | 75.0 % (18 of 24) |
1. CssA5 RNA (43-MER)
GGUGAGUACG UAGAGUAUAC UUCGGUAUAC UUAUAUACUU ACCSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CssA5 RNA (43-MER) | natural abundance | 0.4 ~ 1.1 mM | |
2 | CssA5 RNA (43-MER) | 13C/15N-uniformly labeled | 0.4 ~ 1.1 mM | |
3 | CssA5 RNA (43-MER) | 13C/15N-AU labeled | 0.4 ~ 1.1 mM | |
4 | CssA5 RNA (43-MER) | partially deuterated | 0.4 ~ 1.1 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |