BMRBj - BMREntryMaster

Found 1 Document (0.481 seconds)

BMRB: 34713

7Z0B

Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L and G26L double mutant TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155)

Entity

1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155) (polymer, Thiol state: not present), 29 monomers, 3656.578 Da Detail

KKKLGFFLVT FLFIWLMMLL HFTIQQRKK

Formula weight

3656.578 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 65.3 %, Completeness (bb): 71.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.3 % (277 of 424)	67.1 % (149 of 222)	59.2 % (100 of 169)	84.8 % (28 of 33)
Backbone	71.8 % (125 of 174)	78.0 % (46 of 59)	60.5 % (52 of 86)	93.1 % (27 of 29)
Sidechain	61.2 % (170 of 278)	60.7 % (99 of 163)	63.1 % (70 of 111)	25.0 % (1 of 4)
Aromatic	45.5 % (30 of 66)	51.5 % (17 of 33)	37.5 % (12 of 32)	100.0 % (1 of 1)
Methyl	95.0 % (38 of 40)	95.0 % (19 of 20)	95.0 % (19 of 20)

1. entity 1

KKKLGFFLVT FLFIWLMMLL HFTIQQRKK

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 300 K, pH 7.0, Details 500 uM GNTV_G22LG26L, trifluoroethanol/water

#	Name	Isotope labeling	Type	Concentration
1	GNTV_G22LG26L	natural abundance		500 uM

Release date

2022-03-16

Citation

Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage
Papadopoulou, A.A., Stelzer, W., Silber, M., Schlosser, C., Spitz, C., Haug-Kroper, M., Straub, T., Muller, S.A., Lichtenthaler, S.F., Muhle-Goll, C., Langosch, D., Fluhrer, R.
Sci. Rep. (2022), 12, 20987-20987, PubMed 36470941 , DOI 10.1038/s41598-022-24772-8 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 34710 released on 2022-03-22
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) TMD
  BMRB: 34711 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G26P mutant TMD
  BMRB: 34712 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L mutant TMD

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34713_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34713_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	324		96.6 (chain: 1, length: 29)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 29, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 196
  - ¹³C chemical shifts: 99
  - ¹⁵N chemical shifts: 29
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	222	160	72.1
¹³C chemical shifts	169	99	58.6
¹⁵N chemical shifts	33	29	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	56	94.9
¹³C chemical shifts	58	28	48.3
¹⁵N chemical shifts	29	28	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	163	104	63.8
¹³C chemical shifts	111	71	64.0
¹⁵N chemical shifts	4	1	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	18	81.8
¹³C chemical shifts	22	18	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	19	57.6
¹³C chemical shifts	32	13	40.6
¹⁵N chemical shifts	1	1	100.0

Keywords Glycosyltransferase, GnTV, Golgi, MEMBRANE PROTEIN, SPPL3, TMD, intramembrane

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Found 1 Document (0.481 seconds)

BMRB: 34713

Sample

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail