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BMRB: 16230

2KH9

Solution Structure of Yeast Prp24-RRM2 Bound to a Fragment of U6 RNA

Authors

Martin-Tumasz, S.A., Butcher, S.E.

Assembly

U6 snRNP (small fragment)

Entity

1. Prp24-RRM2 (polymer, Thiol state: not reported), 92 monomers, 10824.24 Da Detail

MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLELEHHHH HH

2. AGAGAU (polymer, Thiol state: not present), 6 monomers, 2002.210 Da Detail

AGAGAU

Total weight

12826.45 Da

Max. entity weight

10824.24 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS61:SG

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 84.4 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	84.4 % (1013 of 1200)	88.3 % (551 of 624)	77.9 % (371 of 476)	91.0 % (91 of 100)
Backbone	91.1 % (556 of 610)	92.7 % (202 of 218)	89.1 % (271 of 304)	94.3 % (83 of 88)
Sidechain	79.6 % (541 of 680)	86.0 % (349 of 406)	70.2 % (184 of 262)	66.7 % (8 of 12)
Aromatic	33.9 % (42 of 124)	67.7 % (42 of 62)	0.0 % (0 of 58)	0.0 % (0 of 4)
Methyl	90.0 % (99 of 110)	90.9 % (50 of 55)	89.1 % (49 of 55)

1. Prp24-RRM2

MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLELEHHHH HH

2. AGAGAU

AGAGAU

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
1	Prp24-RRM2	natural abundance	300 uM
2	AGAGAU	natural abundance	3 mM
3	TRIS	[U-2H]	10 mM
4	potassium chloride	natural abundance	50 mM
5	D20	natural abundance	100 %

Sample #2

Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
6	Prp24-RRM2	[U-99% 15N]	500 uM
7	AGAGAU	natural abundance	5 mM
8	TRIS	[U-2H]	10 mM
9	potassium chloride	natural abundance	50 mM
10	D20	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
18	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 uM
19	AGAGAU	natural abundance	5 mM
20	TRIS	natural abundance	10 mM
21	potassium chloride	natural abundance	50 mM
22	DTT	natural abundance	1 mM
23	DSS	natural abundance	10 uM
24	H20	natural abundance	90 %
25	D20	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
26	Prp24-RRM2	[U-99% 13C; U-99% 15N]	300 uM
27	AGAGAU	natural abundance	3 mM
28	TRIS	natural abundance	10 mM
29	potassium chloride	natural abundance	50 mM
30	DTT	natural abundance	1 mM
31	DMPC/DHPC 3:1	natural abundance	6.5 %
32	H20	natural abundance	90 %
33	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KH9, Strand ID: A Detail

Release date

2009-09-03

Citation

(1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA
Martin-Tumasz, S., Butcher, S.E.
Biomol. NMR Assign. (2009), 3, 227-230, PubMed 19693704 , DOI 10.1007/s12104-009-9181-5 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 16243 released on 2009-09-03
    Title Backbone NH Assignments for Prp24-RRM23
  BMRB: 16244 released on 2009-09-03
    Title Backbone NH Assignments for Prp24-RRM23 Bound to AGAGAU RNA
  BMRB: 16246 released on 2009-09-03
    Title Resonance Assignments for Yeast Prp24-RRM2

Related entities 1. Prp24-RRM2, : 1 : 99 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-13C HSQC

Instrument

Varian DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
18	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 uM
19	AGAGAU	natural abundance	5 mM
20	TRIS	natural abundance	10 mM
21	potassium chloride	natural abundance	50 mM
22	DTT	natural abundance	1 mM
23	DSS	natural abundance	10 uM
24	H20	natural abundance	90 %
25	D20	natural abundance	10 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

3 2D 1H-13C HSQC

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
6	Prp24-RRM2	[U-99% 15N]	500 uM
7	AGAGAU	natural abundance	5 mM
8	TRIS	[U-2H]	10 mM
9	potassium chloride	natural abundance	50 mM
10	D20	natural abundance	100 %

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

5 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

6 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

8 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

10 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

11 3D 1H-13C NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
6	Prp24-RRM2	[U-99% 15N]	500 uM
7	AGAGAU	natural abundance	5 mM
8	TRIS	[U-2H]	10 mM
9	potassium chloride	natural abundance	50 mM
10	D20	natural abundance	100 %

12 2D 1H-1H NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
6	Prp24-RRM2	[U-99% 15N]	500 uM
7	AGAGAU	natural abundance	5 mM
8	TRIS	[U-2H]	10 mM
9	potassium chloride	natural abundance	50 mM
10	D20	natural abundance	100 %

13 2D 1H-1H NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
1	Prp24-RRM2	natural abundance	300 uM
2	AGAGAU	natural abundance	3 mM
3	TRIS	[U-2H]	10 mM
4	potassium chloride	natural abundance	50 mM
5	D20	natural abundance	100 %

14 2D 1H-15N HSQC-IPAP

Instrument

Varian DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

15 2D 1H-15N HSQC-IPAP

Instrument

Varian DMX - 750 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
26	Prp24-RRM2	[U-99% 13C; U-99% 15N]	300 uM
27	AGAGAU	natural abundance	3 mM
28	TRIS	natural abundance	10 mM
29	potassium chloride	natural abundance	50 mM
30	DTT	natural abundance	1 mM
31	DMPC/DHPC 3:1	natural abundance	6.5 %
32	H20	natural abundance	90 %
33	D20	natural abundance	10 %

16 3D J modulated CHSQC

Instrument

Varian DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
11	Prp24-RRM2	[U-99% 13C; U-99% 15N]	500 mM
12	AGAGAU	natural abundance	5 mM
13	TRIS	natural abundance	10 mM
14	potassium chloride	natural abundance	50 mM
15	DTT	natural abundance	1 mM
16	H20	natural abundance	90 %
17	D20	natural abundance	10 %

17 3D J modulated CHSQC

Instrument

Varian DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
26	Prp24-RRM2	[U-99% 13C; U-99% 15N]	300 uM
27	AGAGAU	natural abundance	3 mM
28	TRIS	natural abundance	10 mM
29	potassium chloride	natural abundance	50 mM
30	DTT	natural abundance	1 mM
31	DMPC/DHPC 3:1	natural abundance	6.5 %
32	H20	natural abundance	90 %
33	D20	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_16230_2kh9.nef
Input source #2: Coordindates	2kh9.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

----120-------130-------140-------150-------160-------170-------180-------190-------200-----
MTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLELEHHHHHH
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLELEHHHHHH
--------10--------20--------30--------40--------50--------60--------70--------80--------90--

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-50---
AGAGAU
||||||
AGAGAU
------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	92	0	0	100.0
B	B	6	0	0	100.0

Content subtype: combined_16230_2kh9.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1002		95.7 (chain: A, length: 92), 100.0 (chain: B, length: 6)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	Error	2577 (1)	noe	90.2 (chain: A, length: 92), 100.0 (chain: B, length: 6)
2	Distance restraints	CNS/XPLOR_distance_constraints_2	Error	49 (1)	hbond	43.5 (chain: A, length: 92), 50.0 (chain: B, length: 6)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_5	OK	137 (137)	.	77.2 (chain: A, length: 92), 100.0 (chain: B, length: 6)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_6	OK	130 (130)	.	85.9 (chain: A, length: 92)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 95.7%
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 367
  - ¹H chemical shifts: 544
  - ¹⁵N chemical shifts: 91
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	436	345	79.1
¹H chemical shifts	575	501	87.1
¹⁵N chemical shifts	104	91	87.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	184	163	88.6
¹H chemical shifts	182	168	92.3
¹⁵N chemical shifts	88	83	94.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	252	182	72.2
¹H chemical shifts	393	333	84.7
¹⁵N chemical shifts	16	8	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	57	49	86.0
¹H chemical shifts	57	49	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	48	0	0.0
¹H chemical shifts	49	31	63.3
¹⁵N chemical shifts	1	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	40	22	55.0
¹H chemical shifts	49	43	87.8
¹⁵N chemical shifts	3	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	30	22	73.3
¹H chemical shifts	36	33	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	10	0	0.0
¹H chemical shifts	13	10	76.9
¹⁵N chemical shifts	3	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	8	0	0.0
¹H chemical shifts	8	8	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: Error
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 90.2%
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 100.0%
- Total number of restraints: 2526
- Weight of restraints: 1.0 (2526)
- Potential type of restraints: square-well-parabolic (2526)
- Range of distance target values: 2.55, 9.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_2
  - Status: Error
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 43.5%
    - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 50.0%
  - Total number of restraints: 49
  - Weight of restraints: 1.0 (49)
  - Potential type of restraints: square-well-parabolic (49)
  - Range of distance target values: 2.0, 3.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 77.2%
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 100.0%
- Total number of restraints: 137
- Total number of restraints per polymer type: 137
- Weight of restraints: 1.0 (137)
- Potential type of restraints: square-well-parabolic (137)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_6

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 85.9%

----120-------130-------140-------150-------160-------170-------180-------190-------200-----
MTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLELEHHHHHH
  ||||||||| |||||||||||||| |||||||||||||| | |||||||||||||||||||||||||||||||||||| |||||      
..ECTLWMTNF.PSYTQRNIRDLLQD.NVVALSIRLPSLRF.T.RRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVS.PLELE      
----120-------130-------140-------150-------160-------170-------180-------190---------

Total number of restraints: 130
Potential type of restraints: undefined (130)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords Protein/RNA, RRM, snRNP, Splicing

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Found 1 Document (0.68 seconds)

BMRB: 16230

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Prp24-RRM2, : 1 : 99 entities Detail

Experiments performed 17 experiments Detail

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NMR combined restraints 6 contents Detail