Backbone NH Assignments for Prp24-RRM23 Bound to AGAGAU RNA
MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLEKSKRTD SATLEGREIM IRNLSTELLD ENLLRESFEG FGSIEKINIP AGQKEHSFNN CCAFMVFENK DSAERALQMN RSLLGNREIS VSLADKKPLE HHHHHH
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 15N | |
---|---|---|---|
All | 19.7 % (277 of 1406) | 12.0 % (144 of 1204) | 65.8 % (133 of 202) |
Backbone | 45.5 % (268 of 589) | 33.0 % (135 of 409) | 73.9 % (133 of 180) |
Sidechain | 1.1 % (9 of 817) | 1.1 % (9 of 795) | 0.0 % (0 of 22) |
Aromatic | 0.0 % (0 of 93) | 0.0 % (0 of 89) | 0.0 % (0 of 4) |
Methyl | 4.0 % (4 of 100) | 4.0 % (4 of 100) |
1. Prp24-RRM23
MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLEKSKRTD SATLEGREIM IRNLSTELLD ENLLRESFEG FGSIEKINIP AGQKEHSFNN CCAFMVFENK DSAERALQMN RSLLGNREIS VSLADKKPLE HHHHHH2. AGAGAU
AGAGAUSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Prp24-RRM23 | [U-99% 13C; U-99% 15N] | 500 uM | |
2 | TRIS | natural abundance | 10 mM | |
3 | potassium chloride | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | AGAGAU | natural abundance | 5 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Prp24-RRM23 | [U-99% 13C; U-99% 15N] | 500 uM | |
9 | TRIS | natural abundance | 10 mM | |
10 | potassium chloride | natural abundance | 50 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | DSS | natural abundance | 10 uM | |
13 | AGAGAU | natural abundance | 5 mM | |
14 | H2O | natural abundance | 90 % | |
15 | D2O | natural abundance | 10 % |