Backbone NH Assignments for Prp24-RRM23
MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLEKSKRTD SATLEGREIM IRNLSTELLD ENLLRESFEG FGSIEKINIP AGQKEHSFNN CCAFMVFENK DSAERALQMN RSLLGNREIS VSLADKKPLE HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 27.1 % (367 of 1354) | 17.8 % (206 of 1155) | 80.9 % (161 of 199) |
Backbone | 60.2 % (333 of 553) | 46.1 % (172 of 373) | 89.4 % (161 of 180) |
Sidechain | 4.2 % (34 of 801) | 4.3 % (34 of 782) | 0.0 % (0 of 19) |
Aromatic | 6.5 % (5 of 77) | 6.6 % (5 of 76) | 0.0 % (0 of 1) |
Methyl | 7.0 % (7 of 100) | 7.0 % (7 of 100) |
1. Prp24-RRM23
MTECTLWMTN FPPSYTQRNI RDLLQDINVV ALSIRLPSLR FNTSRRFAYI DVTSKEDARY CVEKLNGLKI EGYTLVTKVS NPLEKSKRTD SATLEGREIM IRNLSTELLD ENLLRESFEG FGSIEKINIP AGQKEHSFNN CCAFMVFENK DSAERALQMN RSLLGNREIS VSLADKKPLE HHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Prp24-RRM23 | [U-99% 13C; U-99% 15N] | 500 mM | |
2 | TRIS | natural abundance | 10 mM | |
3 | potassium chloride | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H20 | natural abundance | 90 % | |
6 | D20 | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Prp24-RRM23 | [U-99% 13C; U-99% 15N] | 500 uM | |
8 | TRIS | natural abundance | 10 mM | |
9 | potassium chloride | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | DSS | natural abundance | 10 uM | |
12 | H20 | natural abundance | 90 % | |
13 | D20 | natural abundance | 10 % |