Structure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorylated derived peptide.
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 70.5 % (198 of 281) | 70.5 % (198 of 281) |
Backbone | 75.0 % (69 of 92) | 75.0 % (69 of 92) |
Sidechain | 68.3 % (129 of 189) | 68.3 % (129 of 189) |
Aromatic | 62.1 % (18 of 29) | 62.1 % (18 of 29) |
Methyl | 88.2 % (15 of 17) | 88.2 % (15 of 17) |
1. first WW domain of human YAP
GAMEDVPLPA GWEMAKTSSG QRYFLNHIDQ TTTWQDPRKA2. Smad1 doubly-phosphorilated derived peptide
SXYPHXPTSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details Unlabeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NEDD4LWW3 | natural abundance | 1 mM | |
2 | SMAD3 | natural abundance | 3 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 2 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | NEDD4LWW3 | [U-100% 15N] | 1 mM | |
9 | SMAD3 | natural abundance | 3 mM | |
10 | sodium phosphate | natural abundance | 20 mM | |
11 | sodium chloride | natural abundance | 100 mM | |
12 | sodium azide | natural abundance | 2 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 7, Details 15N,13C labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | NEDD4LWW3 | [U-100% 13C; U-100% 15N] | 1 mM | |
16 | SMAD3 | natural abundance | 3 mM | |
17 | sodium phosphate | natural abundance | 20 mM | |
18 | sodium chloride | natural abundance | 100 mM | |
19 | sodium azide | natural abundance | 2 mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |