BMRBj - BMREntryMaster

Found 1 Document (0.503 seconds)

BMRB: 16303

SLAS-bound SaS synuclein variant

Authors

Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.

Assembly

monomer

Entity

1. monomer (polymer, Thiol state: not present), 140 monomers, 14459.97 Da Detail

MDVFMKGLEK TKEQVTNVGS KTKEGVVHGV GAVVTGVTAV AEKTKQGVAE AAATVASKAK EGVVAAAGKT KEGVLYVGQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Formula weight

14459.97 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 45.2 %, Completeness (bb): 79.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.2 % (677 of 1498)	20.0 % (154 of 771)	66.9 % (390 of 583)	92.4 % (133 of 144)
Backbone	79.3 % (658 of 830)	47.4 % (139 of 293)	96.0 % (386 of 402)	98.5 % (133 of 135)
Sidechain	17.3 % (137 of 790)	3.1 % (15 of 478)	40.3 % (122 of 303)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 28)
Methyl	17.7 % (28 of 158)	6.3 % (5 of 79)	29.1 % (23 of 79)

1. SLAS-bound SaS

MDVFMKGLEK TKEQVTNVGS KTKEGVVHGV GAVVTGVTAV AEKTKQGVAE AAATVASKAK EGVVAAAGKT KEGVLYVGQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.2 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	SaS	[U-100% 13C; U-100% 15N; U-80% 2H]	0.75 mM
2	sodium lauroyl sarcosinate	natural abundance	75 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.72 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.72 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Release date

2009-06-02

Citation

Effect of pseudorepeat rearrangement on alpha-synuclein misfolding, vesicle binding, and micelle binding
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
J. Mol. Biol. (2009), 390, 516-529, PubMed 19481090 , DOI 10.1016/j.jmb.2009.05.058 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 16300 released on 2009-06-02
    Title intrinsically unfolded alpha-synuclein
  BMRB: 16301 released on 2009-06-02
    Title intrinsically unfolded alpha-synuclein variant SaS
  BMRB: 16302 released on 2009-06-02
    Title SLAS-bound alpha-synuclein
  BMRB: 16304 released on 2009-06-02
    Title SLAS-bound beta-synuclein

Related entities 1. monomer, : 154 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16303_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16303_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	646		95.0 (chain: 1, length: 140)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 140, Sequence coverage: 95.0%
- Total number of assignments
  - ¹H chemical shifts: 133
  - ¹³C chemical shifts: 380
  - ¹⁵N chemical shifts: 133
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	771	133	17.3
¹³C chemical shifts	583	380	65.2
¹⁵N chemical shifts	144	133	92.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	293	133	45.4
¹³C chemical shifts	280	265	94.6
¹⁵N chemical shifts	135	133	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	478	0	0.0
¹³C chemical shifts	303	115	38.0
¹⁵N chemical shifts	9	0	0.0

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Found 1 Document (0.503 seconds)

BMRB: 16303

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. monomer, : 154 entities Detail

Experiments performed 4 experiments Detail

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Instrument

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Instrument

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Sample

Chemical shift validation 3 contents Detail