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BMRB: 4396


4396
1COU
Anticoagulant protein from the nematode Ancylostoma caninum
Authors
Duggan, B.M., Dyson, H.JANE., Wright, P.E.
Assembly
Nematode Anticoagulant Protein c2
Entity
1. Nematode Anticoagulant Protein c2 (polymer, Thiol state: all disulfide bound), 85 monomers, 9745.618 Da Detail

KATMQCGENE KYDSCGSKEC DKKCKYDGVE EEDDEEPNVP CLVRVCHQDC VCEEGFYRNK DDKCVSAEDC ELDNMDFIYP GTRNP


Formula weight
9745.618 Da
Entity Connection
disulfide 5 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS6:SG1:CYS50:SG
2disulfidesing1:CYS15:SG1:CYS46:SG
3disulfidesing1:CYS20:SG1:CYS41:SG
4disulfidesing1:CYS24:SG1:CYS70:SG
5disulfidesing1:CYS52:SG1:CYS64:SG

Source organism
Ancylostoma caninum
Exptl. method
NMR
Refine. method
MOLECULAR DYNAMICS
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 64.3 %, Completeness (bb): 56.6 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All64.3 % (607 of 944)93.2 % (465 of 499)16.0 % (57 of 357)96.6 % (85 of 88)
Backbone56.6 % (284 of 502)97.7 % (167 of 171)15.6 % (39 of 250)96.3 % (78 of 81)
Sidechain64.8 % (338 of 522)90.9 % (298 of 328)17.6 % (33 of 187)100.0 % (7 of 7)
Aromatic53.6 % (30 of 56)100.0 % (28 of 28) 7.1 % (2 of 28)
Methyl68.2 % (30 of 44)100.0 % (22 of 22)36.4 % (8 of 22)

1. Nematode Anticoagulant Protein c2

KATMQCGENE KYDSCGSKEC DKKCKYDGVE EEDDEEPNVP CLVRVCHQDC VCEEGFYRNK DDKCVSAEDC ELDNMDFIYP GTRNP

Show sample condition
Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.05), Details The 15N label is around 50-60%


#NameIsotope labelingTypeConcentration
1Nematode Anticoagulant Protein c2[U-55% 15N]2.0 mM
2sodium chloride50 mM
3DSS20 uM

Protein Blocks Logo
Calculated from 18 models in PDB: 1COU, Strand ID: A Detail

Coupling constant
76 J values in 1 lists
Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.05) Detail
Release date
2007-06-18
Citation 1
Inherent flexibility in a potent inhibitor of blood coagulation, recombinant nematode anticoagulant protein c2
Duggan, B.M., Dyson, H.JANE., Wright, P.E.
Eur. J. Biochem. (1999), 265, 539-548, PubMed 10504384 , DOI 10.1046/j.1432-1327.1999.00781.x ,
Show more 3 citaions
Citation 2
Anticoagulant repertoire of the hookworm Ancylostoma caninum
Stassens, P., Bergum, P.W., Gansemans, Y., Jespers, L., Laroche, Y., Huang, S., Maki, S., Messens, J., Lauwereys, M., Cappello, M., Hotez, P.J., Lasters, I., Vlasuk, G.P.
Proc. Natl. Acad. Sci. U. S. A. (1996), 93, 2149-2154, PubMed 8700900 , DOI 10.1073/pnas.93.5.2149 ,
Citation 3
Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 ,
Citation 4
Stereochemical quality of protein structure coordinates
Morris, A.L., MacArthur, M.W., Hutchinson, E.G., Thornton, J.M.
Proteins (1992), 12, 345-364, PubMed 1579569 , DOI 10.1002/prot.340120407 ,
Entries sharing articles BMRB: 27 entries Detail
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    Title NMR STRUCTURE OF THE PEPTAIBOL CHRYSOSPERMIN C BOUND TO DPC MICELLES
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  BMRB: 4921 released on 2008-07-15
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  BMRB: 6723 released on 2005-10-13
    Title NMR structure of the heme chaperone CcmE reveals a novel functional motif
  BMRB: 6313 released on 2005-02-07
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  BMRB: 5844 released on 2004-07-25
    Title ZR18 Structure determination
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  BMRB: 5596 released on 2003-09-04
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  BMRB: 5691 released on 2003-02-24
    Title Solution Structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19
  BMRB: 5379 released on 2003-02-19
    Title Bovine Pancreatic Polypeptide Bound to DPC Micelles
  BMRB: 5184 released on 2002-05-07
    Title 1H Chemical Shift Assignments for toxin BeKm from the Scorpion Buthus eupeus
  BMRB: 5275 released on 2002-03-21
    Title The rhesus rotavirus sialic acid binding domain without ligand
  BMRB: 5044 released on 2001-11-13
    Title 1H, 13C, and 15N Chemical shift assignments for the third Immunoglobulin domain from the neural cell adhesion molecule, N-CAM
  BMRB: 4979 released on 2001-11-08
    Title 1H and 13C chemical shift assignments for the pheromone Er-23 from Euplotes raikovi
  BMRB: 4774 released on 2001-05-02
    Title Three-dimensional Structure of the Histidine-containing Phosphocarrier Protein (Hpr) from Enterococcus faecalis in Solution
  BMRB: 4980 released on 2001-03-24
    Title 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease
  BMRB: 4422 released on 2000-12-18
    Title NMR Solution Structure of Apis mellifera Chymotrypsin Inhibitor (AMCI).
  BMRB: 4602 released on 2000-12-05
    Title Solution Structure of Oxidized Microsomal Rabbit Cytochrome b5. Factors Determining the Heterogeneous Binding of the Heme
  BMRB: 4815 released on 2000-11-12
    Title NMR structure of Cardiotoxin in DPC-micelle
  BMRB: 4214 released on 2000-06-16
    Title N-terminal Domain of Tissue Inhibitor of Metalloproteinase-2 (N-TIMP-2)
  BMRB: 4438 released on 2000-03-22
    Title 1H, 13C and 15N chemical Shift Assignments for SCP2
  BMRB: 4429 released on 1999-12-22
    Title 1H and 15N Chemical Shift Assignments for ribosomal protein L7
  BMRB: 4418 released on 1999-11-23
    Title NMR structure with tightly bound water molecules of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution (minor form)
  BMRB: 4419 released on 1999-11-11
    Title NMR structure with tightly bound water molecules of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution (major form)
Related entities 1. Nematode Anticoagulant Protein c2, : 1 : 1 : 26 entities Detail
More details for 28 related entities
Experiments performed 9 experiments Detail
nullKeywords anticoagulant, protease inhibitor