Anticoagulant protein from the nematode Ancylostoma caninum
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS6:SG | 1:CYS50:SG |
2 | disulfide | sing | 1:CYS15:SG | 1:CYS46:SG |
3 | disulfide | sing | 1:CYS20:SG | 1:CYS41:SG |
4 | disulfide | sing | 1:CYS24:SG | 1:CYS70:SG |
5 | disulfide | sing | 1:CYS52:SG | 1:CYS64:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 64.3 % (607 of 944) | 93.2 % (465 of 499) | 16.0 % (57 of 357) | 96.6 % (85 of 88) |
Backbone | 56.6 % (284 of 502) | 97.7 % (167 of 171) | 15.6 % (39 of 250) | 96.3 % (78 of 81) |
Sidechain | 64.8 % (338 of 522) | 90.9 % (298 of 328) | 17.6 % (33 of 187) | 100.0 % (7 of 7) |
Aromatic | 53.6 % (30 of 56) | 100.0 % (28 of 28) | 7.1 % (2 of 28) | |
Methyl | 68.2 % (30 of 44) | 100.0 % (22 of 22) | 36.4 % (8 of 22) |
1. Nematode Anticoagulant Protein c2
KATMQCGENE KYDSCGSKEC DKKCKYDGVE EEDDEEPNVP CLVRVCHQDC VCEEGFYRNK DDKCVSAEDC ELDNMDFIYP GTRNPPressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.05), Details The 15N label is around 50-60%
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nematode Anticoagulant Protein c2 | [U-55% 15N] | 2.0 mM | |
2 | sodium chloride | 50 mM | ||
3 | DSS | 20 uM |