NMR structure of the heme chaperone CcmE reveals a novel functional motif
LRSNIDLFYT PGEILYGKRE TQQMPEVGQR LRVGGMVMPG SVQRDPNSLK VTFTIYDAEG SVDVSYEGIL PDLFREGQGV VVQGELEKGN HILAKEVLAK HDENYTPPEV EKAMEANHRR PASVYKDPAS HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.8 % (1268 of 1570) | 90.0 % (739 of 821) | 65.8 % (402 of 611) | 92.0 % (127 of 138) |
Backbone | 79.6 % (635 of 798) | 92.7 % (255 of 275) | 66.4 % (263 of 396) | 92.1 % (117 of 127) |
Sidechain | 83.4 % (747 of 896) | 88.6 % (484 of 546) | 74.6 % (253 of 339) | 90.9 % (10 of 11) |
Aromatic | 37.7 % (43 of 114) | 75.4 % (43 of 57) | 0.0 % (0 of 57) | |
Methyl | 95.6 % (130 of 136) | 97.1 % (66 of 68) | 94.1 % (64 of 68) |
1. Escherichia coli apo-CcmE
LRSNIDLFYT PGEILYGKRE TQQMPEVGQR LRVGGMVMPG SVQRDPNSLK VTFTIYDAEG SVDVSYEGIL PDLFREGQGV VVQGELEKGN HILAKEVLAK HDENYTPPEV EKAMEANHRR PASVYKDPAS HHHHHHTemperature 303 (±1) K, pH 7.2 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Escherichia coli apo-CcmE | [U-98% 13C; U-98% 15N] | 1.1 mM | |
2 | sodium phosphate | 50 mM | ||
3 | sodium chloride | 300 mM |