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BMRB: 4953

1HXV

Assignment of 1H, 13C and 15N resonances of the PPIase domain of the trigger factor from Mycoplasma genitalium

Authors

Parac, T.N., Vogtherr, M., Marcus, M., Pahl, A., Rueterjans, H., Griesinger, C., Fiebig, K.M.

Assembly

trigger factor PPIase domain

Entity

1. trigger factor PPIase domain (polymer, Thiol state: not present), 113 monomers, 12433.17 Da Detail

MRGSHHHHHH GSEKLAKTKS TMVDVSDKKL ANGDIAIIDF TGIVDNKKLA SASAQNYELT IGSNSFIKGF ETGLIAMKVN QKKTLALTFP SDYHVKELQS KPVTFEVVLK AIK

Formula weight

12433.17 Da

Source organism

Mycoplasma genitalium

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 89.4 %, Completeness: 78.6 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.6 % (1039 of 1322)	75.3 % (515 of 684)	80.7 % (419 of 519)	88.2 % (105 of 119)
Backbone	87.1 % (587 of 674)	88.3 % (204 of 231)	86.1 % (286 of 332)	87.4 % (97 of 111)
Sidechain	72.1 % (544 of 754)	68.7 % (311 of 453)	76.8 % (225 of 293)	100.0 % (8 of 8)
Aromatic	45.7 % (43 of 94)	48.9 % (23 of 47)	42.6 % (20 of 47)
Methyl	93.3 % (125 of 134)	94.0 % (63 of 67)	92.5 % (62 of 67)

1. Peptidyl-prolyl-cis/trans isomerase

MRGSHHHHHH GSEKLAKTKS TMVDVSDKKL ANGDIAIIDF TGIVDNKKLA SASAQNYELT IGSNSFIKGF ETGLIAMKVN QKKTLALTFP SDYHVKELQS KPVTFEVVLK AIK

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Peptidyl-prolyl-cis/trans isomerase	[U-100% 15N]		2.0 mM
2	sodium phosphate			50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.52 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.52 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 4.04 ppm, Outliers: 3 Detail

Coupling constant

76 J values in 1 lists
Temperature 298 (±1) K, pH 6.5 (±0.2) Detail

Release date

2001-06-28

Citation 1

Assignment of the 1H, 13C and 15N resonances of the PPIase domain of the trigger factor from Mycoplasma genitalium
Parac, T.N., Vogtherr, M., Maurer, M., Pahl, A., Rueterjans, H., Griesinger, C., Fiebig, K.M.
J. Biomol. NMR (2001), 20, 193-194, PubMed 11495256 , DOI: , Abstract

Show more 1 citaion

Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 1 citaion

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Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4953_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1161		88.5 (chain: 1, length: 113)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	120	105	87.5
¹H chemical shifts	684	511	74.7
¹³C chemical shifts	519	416	80.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	111	97	87.4
¹H chemical shifts	231	202	87.4
¹³C chemical shifts	226	192	85.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	9	8	88.9
¹H chemical shifts	453	309	68.2
¹³C chemical shifts	293	224	76.5

#	Name	Isotope labeling	Type	Concentration
3	Peptidyl-prolyl-cis/trans isomerase	[U-100% 13C; U-100% 15N]		2.0 mM
4	sodium phosphate			50 mM

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Found 1 Document (2.269 seconds)

BMRB: 4953

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 34 entries Detail

Related entities 1. trigger factor PPIase domain, : 1 : 76 entities Detail

Experiments performed 13 experiments Detail

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