Multiple Modes of Peptide Recognition by the PTB Domain of the cell fate Determinant Numb
GSPGIPDRVP ESSKPHQWQA DEEAVRSATC SFSVKYLGCV EVFESRGMQV CEEALKVLRQ SRRRPVRGLL HVSGDGLRVV DDETKGLIVD QTIEKVSFCA PDRNHERGFS YICRDGTTRR WMCHGFLACK DSGERLSHAV GCAFAVCLER KQRRTRAAAS GFSNMSFEDF P
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.4 % (1708 of 1933) | 91.4 % (924 of 1011) | 83.7 % (626 of 748) | 90.8 % (158 of 174) |
Backbone | 93.7 % (948 of 1012) | 96.3 % (336 of 349) | 92.2 % (460 of 499) | 92.7 % (152 of 164) |
Sidechain | 84.0 % (906 of 1078) | 88.8 % (588 of 662) | 76.8 % (312 of 406) | 60.0 % (6 of 10) |
Aromatic | 34.7 % (52 of 150) | 69.3 % (52 of 75) | 0.0 % (0 of 73) | 0.0 % (0 of 2) |
Methyl | 97.4 % (148 of 152) | 97.4 % (74 of 76) | 97.4 % (74 of 76) |
1. dNumb PTB
GSPGIPDRVP ESSKPHQWQA DEEAVRSATC SFSVKYLGCV EVFESRGMQV CEEALKVLRQ SRRRPVRGLL HVSGDGLRVV DDETKGLIVD QTIEKVSFCA PDRNHERGFS YICRDGTTRR WMCHGFLACK DSGERLSHAV GCAFAVCLER KQRRTRAAAS GFSNMSFEDF PTemperature 303 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | dNumb PTB | [U-95% 13C; U-95% 15N] | 1.2 mM |
Temperature 303 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | dNumb PTB | [U-95% 15N] | 1.2 mM |
Temperature 303 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | dNumb PTB | [U-10% 13C; U-90% 15N] | 1.0 mM |