Sequence-specific 1H, 13C and 15N NMR Assignments of Recombinant Human Interferon Alpha-2a
CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS1:SG | 1:CYS98:SG |
2 | disulfide | sing | 1:CYS29:SG | 1:CYS138:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.9 % (1914 of 2016) | 96.1 % (1013 of 1054) | 93.0 % (729 of 784) | 96.6 % (172 of 178) |
Backbone | 98.7 % (967 of 980) | 99.1 % (327 of 330) | 98.4 % (482 of 490) | 98.8 % (158 of 160) |
Sidechain | 92.6 % (1107 of 1196) | 94.8 % (686 of 724) | 89.6 % (407 of 454) | 77.8 % (14 of 18) |
Aromatic | 79.0 % (139 of 176) | 96.6 % (85 of 88) | 60.5 % (52 of 86) | 100.0 % (2 of 2) |
Methyl | 98.9 % (178 of 180) | 97.8 % (88 of 90) | 100.0 % (90 of 90) |
1. Interferon alpha-2a
CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKETemperature 298 (±0.1) K, pH 3.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Interferon_alpha-2a | 2.0 mM |