Acid/Urea denatured cold shock protein A (CspA)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 32.6 % (255 of 782) | 16.4 % (66 of 402) | 40.2 % (123 of 306) | 89.2 % (66 of 74) |
Backbone | 61.3 % (255 of 416) | 44.6 % (66 of 148) | 61.5 % (123 of 200) | 97.1 % (66 of 68) |
Sidechain | 13.6 % (58 of 426) | 0.0 % (0 of 254) | 34.9 % (58 of 166) | 0.0 % (0 of 6) |
Aromatic | 0.0 % (0 of 84) | 0.0 % (0 of 42) | 0.0 % (0 of 41) | 0.0 % (0 of 1) |
Methyl | 8.1 % (5 of 62) | 0.0 % (0 of 31) | 16.1 % (5 of 31) |
1. Cold shock protein A
MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK GPAAGNVTSLTemperature 293 (±1) K, pH 2.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cold shock protein A | [U-15N;U-13C] | 2 mM | |
2 | Urea | 6 M |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 20.4 % (319 of 1564) | 11.2 % (90 of 804) | 25.0 % (153 of 612) | 51.4 % (76 of 148) |
Backbone | 36.7 % (305 of 832) | 26.7 % (79 of 296) | 37.5 % (150 of 400) | 55.9 % (76 of 136) |
Sidechain | 9.6 % (82 of 852) | 2.2 % (11 of 508) | 21.4 % (71 of 332) | 0.0 % (0 of 12) |
Aromatic | 0.0 % (0 of 168) | 0.0 % (0 of 84) | 0.0 % (0 of 82) | 0.0 % (0 of 2) |
Methyl | 5.6 % (7 of 124) | 0.0 % (0 of 62) | 11.3 % (7 of 62) |
1. Cold shock protein A
MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK GPAAGNVTSLTemperature 293 (±1) K, pH 2.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cold shock protein A | [U-15N;U-13C] | 2 mM | |
2 | Urea | 6 M |