Main-chain Signal Assignment for the PDZ2 Domain from Human Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide from the Fas Receptor
PKPGDIFEVE LAKNDNSLGI SVTGGVNTSV RHGGIYVKAV IPQGAAESDG RIHKGDRVLA VNGVSLEGAT HKQAVETLRN TGQVVHLLLE KGQSPT
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 24.7 % (185 of 749) | 15.4 % (97 of 629) | 73.3 % (88 of 120) |
Backbone | 52.4 % (177 of 338) | 38.5 % (89 of 231) | 82.2 % (88 of 107) |
Sidechain | 1.9 % (8 of 411) | 2.0 % (8 of 398) | 0.0 % (0 of 13) |
Aromatic | 0.0 % (0 of 22) | 0.0 % (0 of 22) | |
Methyl | 0.0 % (0 of 69) | 0.0 % (0 of 69) |
1. second PDZ domain from hPTP1E
PKPGDIFEVE LAKNDNSLGI SVTGGVNTSV RHGGIYVKAV IPQGAAESDG RIHKGDRVLA VNGVSLEGAT HKQAVETLRN TGQVVHLLLE KGQSPT2. C-terminal peptide from the Fas receptor
DSENSNFRNE IQSLVPressure 1 atm, Temperature 293 (±1) K, pH 6.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hPTP1E-Fas receptor complex | [U-15N; U-13C] | 3 mM | |
2 | sodium phosphate buffer | 50 mM | ||
3 | sodium chloride | 150 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |