Backbone Resonances Assignments of Human Adult Hemoglobin in the Carbonmonoxy Form
VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R
VHLTPEEKSA VTALWGKVNV DEVGGEALGR LLVVYPWTQR FFESFGDLST PDAVMGNPKV KAHGKKVLGA FSDGLAHLDN LKGTFATLSE LHCDKLHVDP ENFRLLGNVL VCVLAHHFGK EFTPPVQAAY QKVVAGVANA LAHKYH
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 3:HEM1:FE | 1:HIS87:NE2 |
2 | na | sing | 3:HEM1:FE | 4:CMO1:C |
3 | na | sing | 3:HEM1:FE | 1:HIS87:NE2 |
4 | na | sing | 3:HEM1:FE | 4:CMO1:C |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 27.6 % (898 of 3256) | 17.0 % (282 of 1659) | 35.2 % (460 of 1307) | 53.8 % (156 of 290) |
Backbone | 45.4 % (769 of 1694) | 32.8 % (190 of 580) | 50.4 % (424 of 841) | 56.8 % (155 of 273) |
Sidechain | 13.4 % (246 of 1829) | 8.5 % (92 of 1079) | 20.9 % (153 of 733) | 5.9 % (1 of 17) |
Aromatic | 2.3 % (7 of 310) | 3.9 % (6 of 155) | 0.0 % (0 of 152) | 33.3 % (1 of 3) |
Methyl | 17.7 % (66 of 372) | 16.1 % (30 of 186) | 19.4 % (36 of 186) |
1. globin subunit alpha
VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R2. globin subunit beta
VHLTPEEKSA VTALWGKVNV DEVGGEALGR LLVVYPWTQR FFESFGDLST PDAVMGNPKV KAHGKKVLGA FSDGLAHLDN LKGTFATLSE LHCDKLHVDP ENFRLLGNVL VCVLAHHFGK EFTPPVQAAY QKVVAGVANA LAHKYHTemperature 302.0 (±0.1) K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | globin subunit alpha | [U-2H; U-13C; U-15N] | 1.0 mM | |
2 | globin subunit beta | [U-2H; U-13C; U-15N] | 1.0 mM | |
3 | Na phosphate | 0.1 M | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 26.2 % (1708 of 6512) | 15.5 % (514 of 3318) | 34.0 % (890 of 2614) | 52.4 % (304 of 580) |
Backbone | 44.0 % (1490 of 3388) | 30.6 % (355 of 1160) | 49.5 % (832 of 1682) | 55.5 % (303 of 546) |
Sidechain | 12.0 % (438 of 3658) | 7.3 % (158 of 2158) | 19.0 % (279 of 1466) | 2.9 % (1 of 34) |
Aromatic | 1.1 % (7 of 620) | 1.9 % (6 of 310) | 0.0 % (0 of 304) | 16.7 % (1 of 6) |
Methyl | 13.8 % (103 of 744) | 11.6 % (43 of 372) | 16.1 % (60 of 372) |
1. globin subunit alpha
VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R2. globin subunit beta
VHLTPEEKSA VTALWGKVNV DEVGGEALGR LLVVYPWTQR FFESFGDLST PDAVMGNPKV KAHGKKVLGA FSDGLAHLDN LKGTFATLSE LHCDKLHVDP ENFRLLGNVL VCVLAHHFGK EFTPPVQAAY QKVVAGVANA LAHKYHTemperature 302.0 (±0.1) K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | globin subunit alpha | [U-2H; U-13C; U-15N] | 1.0 mM | |
2 | globin subunit beta | [U-2H; U-13C; U-15N] | 1.0 mM | |
3 | Na phosphate | 0.1 M | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |